Cell culture

Cell culture

We perform in vitro plasmidic transfections, transitory or established, in order to answer our scientific questions in different human cellular models: HEK293 (Human Embryonic Kidney), U2OS (bone osteosarcoma), using the TurboFect reagent (ThermoFisher Scientific, cationic polymer), in small (6 wells plates) or higher (25cm² flasks) volumes. Cell culture is carried out in a level 2 containment laboratory.

When indicated, the expression of markers of transfection (eGFP, mCherry) is checked by fluorescence microscopy (eVOS).

We realise real time cell physiology monitoring (viability, proliferation) throughout cell culture cycle with the xCELLigence technique (Ozyme), based on an extremely sensitive impedance measurement.