We address the fitness of codon usage bias at the cellular and molecular levels; we apply experimental evolution to analyse genotypic changes by means of next generation sequencing, and monitor phenotypic changes through real-time cell monitoring techniques and comparative proteomics.
Our results will help solve the evolutionary puzzle of codon usage bias for viruses causing chronic infections, and will have implications for the development of mathematical models of virus-host interaction, and for the design of therapeutic vaccines to guide the immune response towards the identification and targeting of the main protein species.
Contributors
Antonin Demange, Marion Picard, Fiona Leblay, Fanni Borveto, Laura Byk, Ignacio Bravo